ELISpot Flex: Salmon IFN-γ (ALP)

ELISpot Flex: Salmon IFN‑γ (ALP)

3190-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT36X
Detection mAbMT10X, biotin
Enzyme conjugateStreptavidin-ALP

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Delivery 4-9 business days

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ELISpot plates, white


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting salmon IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Salmon IFN-γ (ALP)
ApplicationELISpot
AnalyteIFN-γ
ReactivitySalmon
SpecificityThis kit is based on a matched pair of mAbs specific for Atlantic salmon IFN-γ.
Cross-reactivityThe mAbs cross-react with IFN-γ from Rainbow trout. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBL (1,000,000 cells/well) from Atlantic salmon were incubated for 48 h in the absence or presence of PMA/ionomycin and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID100329178